Once the item has been delivered, immediately transfer the vials of ADCC Bioassay Effector Cells and Target Cells for long–term storage below –140°C (freezer or liquid nitrogen vapor phase). The cells are sensitive, and care should be taken when handling. For safety reasons do not store cell vials submerged in liquid nitrogen. Store the Low IgG Serum at –20°C. Avoid multiple freeze–thaw cycles. Store the Control Ab, Anti–CD20, at 4°C. Store the Bio–Glo Luciferase Assay Buffer and Luciferase Assay Substrate at –20°C. For optimal performance, use reconstituted Bio–Glo Luciferase Assay Reagent on the day of preparation. However, once reconstituted, you can store Bio–Glo Luciferase Assay Reagent at –20°C for up to six weeks. Store RPMI 1640 Medium at 4°C away from fluorescent light.
