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Fixatives, Prepared, Electron Microscopy Sciences - 15960-01, 2% Paraformaldehyde/2.5% Glutaraldehyde in 0.1 M Sodium Cacodylate Buffer (pH 7.4)

Tos-Lys-chloromethylketone.HCl. TLCK.

4-Abz-Gly-Pro-D-Leu-D-Ala-NHOH.

1-O-Octadecyl-rac-glycerol. Batyl alcohol.

MEGA-10 1g. MEGA-10, 1g, Synonym: Decanoyl-N-methylglucamide, N-Decanoyl-N-methyl-D-glucamine, Molecular Formula: CGGHGGNOG, Cas Registry Number: : 85261-20-7, Storage Condition: -20 +/- 5 degree C.

Accessories for Genomic DNA Purification and Isolation Protocols, Promega - A2241, 10 pack, Wizard SV 96 Lysate Clearing Plates, 10 pack. Wizard® SV 96 Lysate Clearing Plates, 10 pack

Accessories for Genomic DNA Purification and Isolation Protocols, Promega - A2271, 10 pack, Wizard SV 96 Binding Plates, 10 pack. Wizard® SV 96 Binding Plates, 10 pack

Vac-Man 96 Vacuum Manifold, 1 each, Promega - A2291, 1 each, Vac-Man 96 Vacuum Manifold, 1 each

T4 Polynucleotide Kinase Buffer Pack - C1313, 3 x 500 µL, T4 Polynucleotide Kinase Buffer Pack, Kinase 10X Buffer

1.2kb Kanamycin Positive Control RNA, 5ug. Applications: Synthesis of double-stranded cDNA from poly(A)+ RNA. The Universal RiboClone cDNA Synthesis System is designed for efficient and complete synthesis of double-stranded cDNA using poly(A)+ RNA from any source.

ProFection Mammalian Transfection System Calcium Phosphate, 1 system. The ProFection Mammalian Transfection Systems are designed for high efficiency transfer of DNA into mammalian cells. Two complete systems are available.

Beetle Luciferin, Potassium Salt. Luciferase genes from the common firefly (Photinus pyralis) and from other beetles are commonly used as reporter genes for studying transcription regulation in transient assay systems and as markers for stably transformed eukaryotic cells.

pAdVAntage Vector. Store at -20[degree]C. The pAdVAntage; Vector contains the Adenovirus Virus-associated I (VAI) RNA gene insert. This gene has been shown to increase expression of proteins by enhancing translation.

pCI Mammalian Expression Vector. Store at -20[degree]C. The pCI Mammalian Expression Vector is designed for high level, constitutive expression of genes in mammalian cells. The vector is a high copy plasmid in E. coli and the backbone is based on the pGEM series of vectors.

pRL-SV40 Vector. Store vector DNA at -20[degree]C. Store glycerol stock of JM109 at -70[degree]C. The pRL family of Renilla luciferase (Rluc) control reporter vectors is designed for use with the Dual-Luciferase; Reporter Assay System.

pRL-CMV Vector. Store vector DNA at -20[degree]C. Store glycerol stock of JM109 at -70[degree]C. The pRL family of Renilla luciferase (Rluc) control reporter vectors is designed for use with the Dual-Luciferase; Reporter Assay System.

pRL-null Vector. Store vector DNA at -20[degree]C. Store glycerol stock of JM109 at -70[degree]C. The pRL family of Renilla luciferase (Rluc) control reporter vectors is designed for use with the Dual-Luciferase; Reporter Assay System.

CheckMate Mammalian Two-Hybrid System, 1 System, Promega - E2440, 1 system, CheckMate Mammalian Two-Hybrid System, 1 system

Bright-Glo Luciferase Assay System, Promega - E2650, 10 x 100 ml, Bright-Glo Luciferase Assay System, 10 x 100ml

Beta-Glo Assay System, Promega - E4740, 100ml, Beta-Glo Assay System, 100ml

rATP, 100mM. May be stored up to one year at -20[degree]C.

rUTP, 100mM. May be stored up to one year at -20[degree]C.

rGTP, 100mM. May be stored up to one year at -20[degree]C.

rCTP, 100mM. May be stored up to one year at -20[degree]C.

Vector. PGL4.17(Luc2/Neo) Vector, Signaling Pathway Analysis (Minimal Promoter-Driven) Firefly Luciferase, Optimized For Expression In Mammalian Cells, report the activity of a variety of pathways using the optimized luc2 firefly luciferase gene in the PGL4 backbone, size: 20ug

CellTiter 96 Non-Radioactive Cell Proliferation Assay, 5,000 assays. The CellTiter 96 Assay is a collection of qualified reagents that provide a rapid and convenient non-radioactive alternative to [3H]thymidine for determining viable cell number in proliferation assays.

Bioassay Effector Cell. ADCC Bioassay Effector Cell, Propagation Model, Fc receptor-mediated antibody-dependent cell-mediated cytotoxicity (ADCC), important mechanism of action (MOA) by which antibodies target disease cells for elimination, used to measure ADCC utilize primary donor

Caspase Inhibitor Z-VAD-FMK, 20mM, Z-VAD-FMK (carbobenzoxy-valyl-alanyl-aspartyl-[O-methyl]- fluoromethylketone), cell-permeant pan caspase inhibitor that irreversibly binds to the catalytic site of caspase proteases and can inhibit induction of apoptosis, Size:125ul

Caspase-Glo 8 Assay, Promega - G8202, 100ml, Caspase-Glo 8 Assay, 100ml

HaloTag Coumarin Ligand, 30 µl, Promega - G8581, 30 µl, HaloTag Coumarin Ligand, 30microl

HaloTag PEG-Biotin Ligand, 30 µl, Promega - G8591, 30 µl, HaloTag PEG-Biotin Ligand, 30microl

Beads Protein A Magne. Are Magnetic Affinity Beads With High Specificity And High Capacity For Purification Of Immunoglobulins (Igg) From Cell Culture Media, Ascites And Serum Samples.

Formamide, Molecular grade, used for the denaturation of nucleic acids in applications such as hybridization, sequencing gel electrophoresis and electron microscopy, Purity: or equal to99.5%.Copper: or equal to1ppm, Iron: or equal to1ppm, Lead: or equal to1ppm, Size:500ml

Tris-Hydrochloride, Molecular Biology Grade, used in combination with Tris base for preparation of Tris-HCl buffers, Purity: or equal to99.0%, Iron: or equal to5ppm, Calcium: or equal to5ppm, Lead: or equal to1ppm, Magnesium: or equal to1ppm, Size:2, 500g

HEPES buffer. HEPES, Molecular Biology Grade (free acid), biological buffer that functions over a pH range of 6.8 to 8.2.Appearance: White, crystalline powder, Purity: or equal to99.5%, Lead: or equal to5ppm, Iron: or equal to5ppm, Moisture: or equal to0.5%, Size:500g

E. coli S30 Extract System for Circular DNA, 450ul. Exogenously added circular DNA containing appropriate prokaryotic promoter and ribosome binding sites directs the synthesis of proteins in vitro.

E. coli S30 Extract System for Linear Templates, 450ul. Promega's E. coli S30 Extract System for Linear DNA is useful for coupled transcription/translation of linearized prokaryotic templates.

TNT SP6 Coupled Wheat Germ Extract System, 1 system. Promega's TNT Wheat Germ Extract Systems produce proteins in vitro directly from protein-encoding DNA cloned downstream of T3, T7 or SP6 RNA polymerase promoters.

Luciferase SP6 Control DNA, 20ug. Store at -70[degree]C or -20[degree]C. The Luciferase SP6/T7/T3 Control DNAs are used as functional controls in the TNT Coupled Transcription/Translation Systems.

Luciferase T7 Control DNA, 20ug. Store at -70[degree]C or -20[degree]C. The Luciferase SP6/T7/T3 Control DNAs are used as functional controls in the TNT Coupled Transcription/Translation Systems.

TNT T3 Coupled Reticulocyte Lysate System, 1 system. The TNT Coupled Reticulocyte Lysate Systems are eukaryotic in vitro transcription/translation systems developed by Promega.

FluoroTect GreenLys in vitro Translation Labeling System, 40 reactions, Promega - L5001, 40 reactions, FluoroTect GreenLys in vitro Translation Labeling System, 40 reactions

TNT T7/T3 Coupled Reticulocyte Lysate System, 1 system. The TNT Coupled Reticulocyte Lysate Systems are eukaryotic in vitro transcription/translation systems developed by Promega.

TNT T7/SP6 Coupled Wheat Germ Extract System, 1 system. Promega's TNT Wheat Germ Extract Systems produce proteins in vitro directly from protein-encoding DNA cloned downstream of T3, T7 or SP6 RNA polymerase promoters.

Amino Acid Mixture Minus Methionine and Cysteine, 1mM. Store at -70[degree]C. The Amino Acid Mixtures are compatible for use in the Flexi Lysate, TNT Lysate and standard Rabbit Reticulocyte Lysate Systems, as well as in the Wheat Germ Extract and E. coli S30 Systems.

Amino Acid Mixture Minus Methionine, 1mM. Store at -70[degree]C. The Amino Acid Mixtures are compatible for use in the Flexi Lysate, TNT Lysate and standard Rabbit Reticulocyte Lysate Systems, as well as in the Wheat Germ Extract and E. coli S30 Systems.

PNL3.2.NF-KB-RE 20UG. NanoLuc (Nluc) luciferase (19.1kDa) enzyme. For luminescent reporting using furimazine to produce high intensity, glow-type luminescence. The luminescent reaction is ATP-independent and designed to suppress background luminescence for maximal assay sensitivity.

Substrate. NanoBRET Nano-Glo Substrate, Features: Understand Real Biology, Monitor Changes, See Improved Assay Performance, Scale Your Assays, Enjoy Convenience, Applications: Protein:protein interaction analysis, High-throughput screening, Storage: -30 deg C to -10 deg C, Size: 50 ul,

SP6 RNA Polymerase. For labeling in vitro, transcription in vitro. Concentration: 10-20units/uL. Size: 5000 units. Packaged in a tamper evident, clear plastic sealed packet that contains the enzyme tube, its buffer(s) and a lot-specific Promega Product Information insert. 24-month lifetime.

Riboprobe System Buffers, --. Store at -20[degree]C. Buffer components of the single and combination Riboprobe and pGEM Express Systems are available separately. These pre-tested and transcription-qualified reagents help ensure successful in vitro transcription.

Riboprobe System - SP6, 1 system. The Riboprobe Systems are designed for the preparation of high specific activity single-stranded RNA probes or of microgram quantities of RNA transcripts in vitro.

Riboprobe System - T3, 1 system. The Riboprobe Systems are designed for the preparation of high specific activity single-stranded RNA probes or of microgram quantities of RNA transcripts in vitro.

Riboprobe Combination System - T3/T7 RNA Polymerase, 1 system. The Riboprobe Combination Systems are designed for the preparation of high specific activity single-stranded RNA probes or of microgram quantities of RNA transcripts in vitro.

Ligand. HaloTag Succinimidyl Ester (O2) Ligand, contains a reactive iodoacetamide group connected to an alkylchloride separated by an ethylene glycol repeat, rapidly react with sulfhydryl-containing molecules, Storage: -70 deg C, size: 5mg

Ribo m7G Cap Analog, A254 units, Promega - P1712, 25 A254 units, Ribo m7G Cap Analog, 25 A254 units

pGEM-3Z Vector. Store vector at -20[degree]C and host strain at -70[degree]C. The pGEM-3Z Vector can be used as a standard cloning vector and for in vitro transcription. The presence of the gene encoding the lacZ a-peptide allows recombinants to be selected using blue/white color screening. U.S.

pGEM-4Z Vector. Store vector at -20[degree]C and host strain at -70[degree]C. The pGEM-4Z Vector can be used as a standard cloning vector and for in vitro transcription. The presence of the gene encoding the lacZ a-peptide allows recombinants to be selected using blue/white color screening. U.S.

pSP73 Vector. Store vector at -20[degree]C. The pSP72-pSP73 Vectors can be used as standard cloning vectors and also can be used for in vitro transcription from either strand using the SP6 or T7 promoter.

pGEM-5Zf(+) Vector. Store vector at -20[degree]C and host strain at -70[degree]C.

pGEM-7Zf(+) Vector. Store vector at -20[degree]C and host strain at -70[degree]C.

pGEM Express Positive Control Template. Store at -20[degree]C. The pGEM Express Positive Control Template may be used to monitor the pGEM Express Systems and the Riboprobe Systems. The pGEM Express Positive Control Template was constructed by linearizing the pGEMEX-1 Vector with Sca I.

SP6 RNA Polymerase. For labeling in vitro, transcription in vitro. Concentration: 80units/uL. Size: 2500 units. Packaged in a tamper evident, clear plastic sealed packet that contains the enzyme tube, its buffer(s) and a lot-specific Promega Product Information insert. 24-month lifetime.

Ligand. HaloTag Amine (O2) Ligand, contains a reactive iodoacetamide group connected to an alkylchloride separated by an ethylene glycol repeat, rapidly react with sulfhydryl-containing molecules, Storage: -20 deg C, size: 5mg

pUC/M13 Primer, Reverse (17mer), 2ug, 10ug/ml. Store at -20[degree]C. Storage Buffer: Sterile water. The pUC/M13 Primers are designed for sequencing inserts cloned into the M13 vectors and pUC plasmids developed by Messing (1).

EcoR I Restriction Enzyme. Cut site: CTTAA//G. Size: 25000 (H.C.) units. Following assays assure purity or activity: activity determination assay, overdigestion assay, and ligation assay. Blue/white cloning, genome qualified. With a tube of acetylated BSA. Re-assayed every 3-6 months.

Sal I Restriction Enzyme. Cut site: CAGCT//G. Size: 10000 (1x10000) units. Following assays assure purity or activity: activity determination assay, overdigestion assay, and ligation assay. Blue/white cloning, genome qualified. With a tube of acetylated BSA. Re-assayed every 3-6 months.

Sma I (Xma I) Restriction Enzyme. Cut site: GGG//CCC. Size: 5000 (1x5000) units. The following assays assure purity or activity: activity determination assay, overdigestion assay, and ligation assay. Blue/white cloning, genome qualified. With a tube of acetylated BSA. Re-assayed every 3-6 months.

Taq I, Promega. TaqI, Promega - R6155, 10,000u. 10000 U, Taq I. TaqI, 10,000u

Xho I Restriction Enzyme. Recognition/cut site: GAGCT//C. 10000 (1x10000) units. The following assays assure purity or activity: activity determination assay, overdigestion assay, and ligation assay. Blue/white cloning, genome qualified. With a tube of acetylated BSA. Re-assayed every 3-6 months.

Pvu I Restriction Enzyme. Recognition/cut site: GC//TAGC. Size: 500 (1x500) units. Following assays assure purity or activity: activity determination assay, overdigestion assay, and ligation assay. Includes a tube of acetylated BSA. Re-assayed every 3-6 months.

Mlu I Restriction Enzyme. Cut site: A//CGCGT. Size: 1000 units. The following assays assure purity or activity: activity determination assay, overdigestion assay, and ligation assay. Blue/white cloning, genome qualified. With a tube of acetylated BSA. Re-assayed every 3-6 months.

Sgf I Restriction Enzyme. Recognition/cut site: GCGAT//CGC. Size: 250 units. The following assays assure purity or activity: activity determination assay, overdigestion assay, and ligation assay. Genome qualified. Includes a tube of acetylated BSA. Re-assayed every 3-6 months.

4-Core Buffer Pack (Buffers A,B,C and D), 1ml each. Store at -20[degree]C. The 4-Core Buffer Pack contains convenient aliquots of the restriction enzyme buffers commonly supplied with Promega Restriction Enzymes. The 4-Core Buffer Pack supplies Buffers A-D as 10X solutions.

AttoPhos® AP Fluorescent Substrate System, Promega - S1001, 1 x 36mg, AttoPhos AP Fluorescent Substrate System Trial Size, 1 x 36mg

AttoPhos Substrate. 36MG

AttoPhos Substrate. 100MG

AttoPhos Buffer. 60ML

AttoPhos Buffer. 240ML

Coelenterazine-h, 250microg, Promega - S2011, 250 µg, Coelenterazine-h, 250microg

Anti-Human IgG (H+L), AP Conjugate, 100ul. Promega's secondary antibodies are used in the detection of proteins in Western blotting and ELISAs. The alkaline phosphatase catalyzes colorimetric reactions using Promega's Western Blue Substrate or standard NBT/BCIP.

dUTP, 40 µmol, Promega - U1191, 40 µmol, dUTP, 40micromol

dATP. Store at -20[degree]C. When stored properly, the dNTPs are stable for at least one year. High quality deoxynucleotide triphosphates (dNTPs) are critical for the success of many key procedures such as cDNA synthesis, sequencing and labeling.

dATP, 200 µmol, Promega - U1202, 200 µmol, dATP, 200micromol

dGTP. Store at -20[degree]C. When stored properly, the dNTPs are stable for at least one year. High quality deoxynucleotide triphosphates (dNTPs) are critical for the success of many key procedures such as cDNA synthesis, sequencing and labeling.

dGTP, 200 µmol, Promega - U1212, 200 µmol, dGTP, 200micromol

dCTP. Store at -20[degree]C. When stored properly, the dNTPs are stable for at least one year. High quality deoxynucleotide triphosphates (dNTPs) are critical for the success of many key procedures such as cDNA synthesis, sequencing and labeling.

dCTP, 200 µmol, Promega - U1222, 200 µmol, dCTP, 200micromol

dTTP, 200 µmol, Promega - U1232, 200 µmol, dTTP, 200micromol

Set of dATP, dCTP, dGTP, dTTP, Promega - U1335, 10 µmol each, Set of dATP, dCTP, dGTP, dUTP

LY 294002 [2-(4-Morpholinyl)-8-phenyl-4 H-1-benzopyran-4-one], potent and specific cell-permeable inhibitor of phosphatidylinositol 3-kinases with IC50 values in the 1-50uM range, chemical identity and purity of LY 294002 is confirmed by proton NMR and elemental analysis, size: 5mg

Assay. Contains human recombinant MAO-A enzyme expressed in yeast, Features: Complete Solution, Speed, Simplified Method, Greater Sensitivity, No Fluorescence Interference, Stable Signal, Applications: Measure recombinant MAO activity, Storage: -70 deg C, size: 200 assays

Assay. Contains human recombinant MAO-A enzyme expressed in yeast, Features: Complete Solution, Speed, Simplified Method, Greater Sensitivity, No Fluorescence Interference, Stable Signal, Applications: Measure recombinant MAO activity, Storage: -70 deg C, size: 1, 000 assays

Elastase 5mg. It is a serine protease that preferentially cleaves at the C-terminus of alanine, valine, serine, glycine, leucine or isoleucine. Elastase has a unique capability of digesting elastin. This enzyme can be used alone or in combination with other proteases for protein analysis.

SoftLink Soft Release Avidin Resin, 1ml. Applications: Purification of biotinylated antibodies and other proteins. Purification of in vivo biotinylated fusion proteins from PinPoint; Vectors.

SoftLink Soft Release Avidin Resin, 5ml. Applications: Purification of biotinylated antibodies and other proteins. Purification of in vivo biotinylated fusion proteins from PinPoint; Vectors.

ProteasMAX Surfactant. Trypsin Enhancer is designed to enhance the enzymatic performance of trypsin or chymotrypsin in any digestion reaction performed with the objective of characterizing the products by mass spectrometry or liquid chromatography.

Molecular Grade. Bisacrylamide, Molecular Grade, cross-linking agent used in the preparation of polyacrylamide gels, tested for its efficiency in gel polymerization, Form: White, free-flowing crystals, Properties: Purity, Acrylic Acid, A290, Magnesium, Conductivity (2% in water, size: 125g

Urea, 99% Pure. Store at 22-25[degree]C. Protect from moisture. Urea is a protein denaturant. Urea is qualified for use as the denaturing component in polyacrylamide gels. Each lot is tested and certified to be free of DNase, RNase, and protease activity.

IPTG, 99% Pure. Store dry at 4[degree]C or -20[degree]C. IPTG (isopropyl-b-thiogalactopyranoside) is an inducer of b-galactosidase activity in many bacteria. Functioning as a lac analog, IPTG binds and inhibits the lac repressor b-galactosidase activity.

Tbk1 Kinase Enzyme System Sf9 Insect Cell 1 mg. Tbk1 Kinase Enzyme Easily Screen and Profile TBK1 Kinase Inhibitors, Includes kinase, substrate and reaction buffer, Use with ADP-Glo Assay for bioluminescent detection of kinase activity, Size: 1mg

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